HitHunter® Human NPR1 cGMP Stable Cell Line Assay (CHO-K1)

Background Information:

The HitHunter Human NPR1 cGMP Stable Cell Line Assay (CHO-K1) utilizes the NPR1 cGMP Cell Line. This cell based assay is developed to study drug candidates (small molecules or biologics) targeting NPR1. This kit assesses ligand (e.g. ANP) based stimulation of receptor activity via detection of resulting increases in the levels of intracellular cGMP. This product uses EFC technology. The included cell line overexpresses untagged NPR1, and upon agonist-based activation of the pathway, resulting increases in levels of intracellular cGMP lead to production of increased EFC signal when the cGMP detection kit is applied.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 6‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Signaling Pathway

Family:

Guanylyl Cyclase

Sub Family:

Cyclic Nucleotide Signaling

Protein Name:

Atrial Natriuretic Factor (ANF)

Protein Aliases:

guanylate cyclase A

Accession Number:

NM_000906.3

UniProt Number:

P16066

Gene Name:

NPR1

Gene ID:

4881

Gene Aliases:

GUCY2A|ANPa

Target Species:

Human

Cell Background:

CHO-K1