PathHunter® Human Blk Kinase Activity Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human Blk Kinase Activity Cell-Based Assay Kit (U2OS) utilizes the U2OS BLK Activity Assay Cell Line. The stable engineered cell line included is intended for use in studying small molecule drug candidates targeting cytosolic kinases. The included cell line overexpresses an engineered fusion of the cytokine receptor binding domain (CRBD) of JAK2 (Janus Kinase 2) to the catalytic domain from the kinase of interest, BLK. It also overexpresses EA-tagged SH2 domain protein alongside a PK-tagged relevant CTK receptor, in this case GHR. Upon activation of the GHR, the CRBD-BLK fusion phosphorylates the CTK receptor, leading to SH2-EA recruitment and production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

TK

Sub Family:

Protein Tyrosine

Protein Name:

BLK

Protein Aliases:

B lymphocyte kinase|p55-Blk

Accession Number:

NM_000163

UniProt Number:

P51451

Gene Name:

BLK

Gene ID:

640

Gene Aliases:

MGC10442

Target Species:

Human

Cell Background:

U2OS