PathHunter® Human FGFR3/FGFR3 Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human FGFR3/FGFR3 Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS FGFR3/FGFR3 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. FGF18)-induced dimerization of FGFR3/FGFR3. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress one construct containing FGFR3 fused to PK and another construct containing FGFR3 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

FGFR3

Protein Aliases:

CD antigen CD333

Accession Number:

NM_000142.4

UniProt Number:

P22607

Gene Name:

FGFR3

Gene ID:

2261

Gene Aliases:

CEK2|JTK4|CD333

Target Species:

Human

Cell Background:

U2OS