PathHunter® Human IL-1 alpha (IL1R1/IL1RAP) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human IL-1 alpha (IL1R1/IL1RAP) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS IL1R1/IL1RAP Dimerization Cell Line. This assay enables the assessment of ligand (e.g. IL-1α)-induced dimerization of IL1R1/IL1RAP pairs. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress IL1R1 fused to PK and IL1RAP fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

IL-1 Receptor

Protein Name:

IL1R1

Protein Aliases:

IL-1RT-1|IL-1RT1|CD121 antigen-like family member A|Interleukin-1 receptor alpha|IL-1R-alpha|Interleukin-1 receptor type I|p80|CD antigen CD121a [Cleaved into: Interleukin-1 receptor type 1, membrane form|mIL-1R1|mIL-1RI; Interleukin-1 receptor type 1, soluble form|sIL-1R1|sIL-1RI]

Accession Number:

NM_000877.3

UniProt Number:

P14778

Gene Name:

IL1R1

Gene ID:

3554

Gene Aliases:

IL1R|IL1RA|IL1RT1

Target Species:

Human

Cell Background:

U2OS