PathHunter® Human TSHR(L) β-Arrestin-1 Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human TSHR(L) β-Arrestin-1 Cell-Based Assay Kit (U2OS) utilizes the Human TSHR (Long isoform) β-Arrestin 1 cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., TSH) based activation of TSHR(L) GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged TSHR (Long isoform) target receptor and an EA-tagged β-Arrestin 1 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Glycoprotein Hormone

Sub Family:

Class A

Coupling:

Gs

Protein Name:

TSH

Protein Aliases:

TSH-R

Accession Number:

NM_000369.2

UniProt Number:

P16473

Gene Name:

TSHR

Gene ID:

7253

Gene Aliases:

LGR3

Target Species:

Human

Cell Background:

U2OS