PathHunter® CCR5 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® CCR5 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. CCL3/MIP-1α) based internalization of CCR5 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CCR5. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CCR5-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR5

Protein Aliases:

CC-CKR-5|CCR-5|CCR5|CHEMR13|HIV-1 fusion coreceptor|CD antigen CD195

Accession Number:

BC038398

UniProt Number:

P51681

Gene Name:

CCR5

Gene ID:

1234

Gene Aliases:

CKR-5|CC-CKR-5|CKR5|CD195|IDDM22

Target Species:

Human

Cell Background:

U2OS