Biological Information

Background Information:

The PathHunter® CXCR7 (CMKOR1) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL12/SDF-1α) based activation of CXCR7 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CXCR7 and EA-tagged β-Arrestin 2. Activation of CXCR7 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

ACKR3 (CXCR7)

Protein Aliases:

CXC-R7|CXCR-7|Chemokine orphan receptor 1|G-protein coupled receptor 159|G-protein coupled receptor RDC1 homolog|RDC-1

Accession Number:

NM_020311.2

UniProt Number:

P25106

Gene Name:

ACKR3

Gene ID:

57007

Gene Aliases:

CMKOR1|CXCR7|GPR159|RDC1

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®