Biological Information
Background Information:
The PathHunter® MRGPRD cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged MRGPRD and EA-tagged β-Arrestin 2. Activation of MRGPRD stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Class A Orphan
Sub Family:
Orphan and other 7TM
Coupling:
Gq
Protein Name:
MrgD
Protein Aliases:
G-protein coupled receptor TGR7
Accession Number:
NM_198923
UniProt Number:
Q8TDS7
Gene Name:
MRGPRD
Gene ID:
116512
Gene Aliases:
mrgD
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®