Biological Information
Background Information:
The PathHunter® GPR120L Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. free fatty acids) based internalization of GPR120L via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged GPR120L and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GPR120L receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Free Fatty Acid
Sub Family:
Class A
Protein Name:
FFA4 (GPR120)
Protein Aliases:
G-protein coupled receptor 129|G-protein coupled receptor GT01|G-protein coupled receptor PGR4|Omega-3 fatty acid receptor 1
Accession Number:
NM_181745
UniProt Number:
Q5NUL3
Gene Name:
FFAR4
Gene ID:
338557
Gene Aliases:
PGR4
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®