PathHunter® GRPR Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® GRPR Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. gastrin-releasing peptide) based internalization of GRPR via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged GRPR. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting GRPR-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Bombesin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

BB2

Protein Aliases:

bombesin receptor 2

Accession Number:

NM_005314

UniProt Number:

P30550

Gene Name:

GRPR

Gene ID:

2925

Gene Aliases:

BB2|BB2R

Target Species:

Human

Cell Background:

U2OS