PathHunter® HTR2C (INI) Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® HTR2C Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based internalization of HTR2C via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged HTR2C. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting HTR2C-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

5-HT2C

Protein Aliases:

5-HT2C|5-HTR2C|5-hydroxytryptamine receptor 1C|5-HT-1C|5-HT1C|Serotonin receptor 2C

Accession Number:

NM_000868.2

UniProt Number:

P28335

Gene Name:

HTR2C

Gene ID:

3358

Gene Aliases:

5-HT2C|5HTR2C

Target Species:

Human

Cell Background:

U2OS