PathHunter® PTHR1 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® PTHR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. PTH) based internalization of PTHR1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged PTHR1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting PTHR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Parathyroid Hormone

Sub Family:

Class B

Coupling:

Gq & Gs

Protein Name:

PTH1

Protein Aliases:

PTH/PTHr receptor|Parathyroid hormone 1 receptor|PTH1 receptor

Accession Number:

AY449732

UniProt Number:

Q03431

Gene Name:

PTH1R

Gene ID:

5745

Target Species:

Human

Cell Background:

U2OS