PathHunter® eXpress CXCR6 Total GPCR Internalization Assay

Background Information:

The PathHunter® CXCR6 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CXCL16) based internalization of CXCR6 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged CXCR6 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CXCR6 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CXCR6

Protein Aliases:

CXCR-6|CDw186|G-protein coupled receptor STRL33|G-protein coupled receptor bonzo|CD antigen CD186

Accession Number:

AY322543

UniProt Number:

O00574

Gene Name:

CXCR6

Gene ID:

10663

Gene Aliases:

TYMSTR|STRL33|BONZO|CD186

Target Species:

Human

Cell Background:

U2OS