Biological Information
Background Information:
The PathHunter® LTB4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged LTB4R and EA-tagged β-Arrestin-2. Activation of LTB4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Leukotriene
Sub Family:
Class A
Coupling:
Gi/Go & Gq
Protein Name:
BLT1 (LTB4)
Protein Aliases:
LTB4-R1|Chemoattractant receptor-like 1|G-protein coupled receptor 16|P2Y purinoceptor 7|P2Y7
Accession Number:
AY322535
UniProt Number:
Q15722
Gene Name:
LTB4R
Gene ID:
1241
Gene Aliases:
BLT|BLT1|BLTR|CMKRL1|GPR16|P2RY7
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®