Ready-to-Assay CXCR2 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CXCR2 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CXCR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CXCR2 (IL-8RB)

Protein Aliases:

CXCR-2|CDw128b|GRO/MGSA receptor|High affinity interleukin-8 receptor B|IL-8R B|IL-8 receptor type 2|CD antigen CD182

Accession Number:

M73969

UniProt Number:

P25025

Gene Name:

CXCR2

Gene ID:

3579

Gene Aliases:

CMKAR2|CD182

Target Species:

Human

Cell Background:

Chem-1