Ready-to-Assay GPR120 Receptor Frozen Cells

Background Information:

The Ready-to-Assay GPR120 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. [ligand]) based activation of FFAR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress FFAR4 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR4 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Free Fatty Acid

Sub Family:

Class A

Protein Name:

FFA4 (GPR120)

Protein Aliases:

G-protein coupled receptor 129|G-protein coupled receptor GT01|G-protein coupled receptor PGR4|Omega-3 fatty acid receptor 1

Accession Number:

BC101175.2

UniProt Number:

Q5NUL3

Gene Name:

FFAR4

Gene ID:

338557

Gene Aliases:

PGR4

Target Species:

Human

Cell Background:

HEK293