Biological Information

Background Information:

The SPRINTer™ eXpress IKZF1 Protein Turnover Biosensor Assay (K562) provides an easy-to-use cell based assay to monitor drug-induced protein turnover of a specific endogenous target. Genome engineering or editing techniques, such as CRISPR/Cas9, are used to introduce a small tag into the endogenous locus of interest. This allows for the quantitation of changes in target protein levels when cells are treated with therapeutic agents such as targeted protein degraders. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Signaling Pathway

Protein Name:

Ikaros

Protein Aliases:

DNA-binding protein Ikaros | Ikaros family zinc finger protein 1|Lymphoid transcription factor LyF-1|protein phosphatase 1, regulatory subunit 92

Accession Number:

Q13422

UniProt Number:

Q13422

Gene Name:

IKZF1

Gene ID:

10320

Gene Aliases:

IK1|IKAROS|LYF1|ZNFN1A1|hIk-1|LyF-1|Hs.54452|PPP1R92

Target Species:

Human

Cell Background:

K562

Usage

Product Type:

eXpress Assay Kits

Application:

Target Engagement

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are unsuitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Visit discoverx.com.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Targeted Protein Degradation (TPD)

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Additional Information

Brand:

SPRINTer™