cAMP Hunter™ CHO-K1 HTR1A Gi Cell Line

Background Information:

The cAMP Hunter™ HTR1A Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR1A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HTR1A , a naturally Gi/Go coupled GPCR. Upon activation of HTR1A , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

5-HT1A

Protein Aliases:

5-HT1A|G-21|Serotonin receptor 1A

Accession Number:

NM_000524.2

UniProt Number:

P08908

Gene Name:

HTR1A

Gene ID:

3350

Gene Aliases:

ADRB2RL1|ADRBRL1

Target Species:

Human

Cell Background:

CHO-K1