Biological Information
Background Information:
The cAMP Hunter™ GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR109A, a naturally Gi/Go coupled GPCR. Upon activation of GPR109A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Hydroxycarboxylic Acid
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
GPR109A
Protein Aliases:
niacin receptor 1|HCA2
Accession Number:
NM_177551
UniProt Number:
Q8TDS4
Gene Name:
HCAR2
Gene ID:
338442
Gene Aliases:
HCA2|HM74A|PUMAG|Puma-g|NIACR1
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Additional Information
Brand:
cAMP Hunter™