cAMP Hunter™ Human CRTH2 Gi Cell-Based Assay Kit (CHO-K1)

Background Information:

The cAMP Hunter™ Human CRTH2 Gi Cell-Based Assay Kit (CHO-K1) utilizes the Human CRTH2 cAMP cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of cAMP signaling following receptor activation for drug discovery and development programs. This cell line enables assessment of ligand (e.g. prostaglandin D2) based activation of CRTH2 GPCR activity via detection of decreasing levels of intracellular cAMP. This assay utilizes EFC technology to measure ligand-induced activation by quantifying intracellular cAMP levels. The cells overexpress wild type CRTH2, a naturally Gi/Go coupled GPCR. Upon activation of CRTH2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This product leverages a sensitive chemiluminescent cAMP detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

DP2

Protein Aliases:

chemoattractant receptor homologous molecule expressed on T helper type 2 cells|G-protein coupled receptor 44|CD antigen CD294

Accession Number:

AY507142

UniProt Number:

Q9Y5Y4

Gene Name:

PTGDR2

Gene ID:

11251

Gene Aliases:

CRTH2|CD294|DP2|DL1R|GPR44

Target Species:

Human

Cell Background:

CHO-K1