ChemiBRITE EP3 Prostanoid Receptor Stable Cell Line

Background Information:

The ChemiBRITE EP3 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3 , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Prostanoid

Sub Family:

Class A

Protein Name:

EP3

Protein Aliases:

PGE2 receptor EP3 subtype|PGE2-R|Prostanoid EP3 receptor

Accession Number:

NM_198716

UniProt Number:

P43115

Gene Name:

PTGER3

Gene ID:

5733

Gene Aliases:

EP3

Target Species:

Human

Cell Background:

HEK293