ChemiSCREEN™ OX2 Orexin Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ OX2 Orexin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. orexins) based activation of HCRTR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HCRTR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HCRTR2 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Orexin

Sub Family:

Class A

Protein Name:

OX2

Protein Aliases:

orexin receptor 2

Accession Number:

NM_001526

UniProt Number:

O43614

Gene Name:

HCRTR2

Gene ID:

3062

Gene Aliases:

OX2R

Target Species:

Human

Cell Background:

Chem-1