Biological Information
Background Information:
The ChemiSCREEN™ PTH1 Parathyroid Hormone Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. parathyroid hormone) based activation of PTH1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTH1R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTH1R , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Parathyroid Hormone
Sub Family:
Class B
Protein Name:
PTH1
Protein Aliases:
PTH/PTHr receptor|Parathyroid hormone 1 receptor|PTH1 receptor
Accession Number:
NM_000316
UniProt Number:
Q03431
Gene Name:
PTH1R
Gene ID:
5745
Target Species:
Human
Cell Background:
Chem-1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
ChemiSCREEN™