Biological Information

Background Information:

The F2RL1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serine proteases, SLIGRL-NH2) based activation of F2RL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses F2RL1 , a naturally Gq coupled GPCR. Upon activation of F2RL1 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Proteinase-Activated

Sub Family:

Class A

Coupling:

Gi/Go & Gq

Protein Name:

PAR2

Protein Aliases:

proteinase-activated receptor-2

Accession Number:

NM_005242.4

UniProt Number:

P55085

Gene Name:

F2RL1

Gene ID:

2150

Gene Aliases:

PAR2|GPR11

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Calcium Flux

Detection Method:

Chemiluminescence

Additional Information

Brand:

Eurofins DiscoverX®