InCELL Hunter™ U2OS MCL1-BIM Protein Binding Cell Line

Background Information:

The InCELL Hunter™ U2OS MCL1-BIM Protein Binding Cell Line provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of MCL1-BIM binding via detection of BIM levels. This product uses EFC technology. The cell line included overexpresses MCL1 target in an untagged form and BIM binding partner fused to PL. When a compound engages the MCL1 target protein, partner binding is disrupted and the destabilized BIM is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Cytosolic (Other)

Family:

Bcl-2

Sub Family:

Apoptosis

Protein Name:

MCL1

Protein Aliases:

Bcl2-L-3|Bcl-2-related protein EAT/mcl1|mcl1/EAT

Accession Number:

NM_021960

UniProt Number:

Q07820

Gene Name:

MCL1

Gene ID:

4170

Gene Aliases:

BCL2L3|Mcl-1

Target Species:

Human

Cell Background:

U2OS