Biological Information
Background Information:
The PathHunter® GPR30 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR30 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged GPR30 and EA-tagged β-Arrestin 2. Activation of GPR30 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Orphan receptor with unknown ligand.
Target Class:
GPCR
Family:
G protein-coupled Estrogen
Sub Family:
Class A
Coupling:
Gi/Go & Gs
Protein Name:
GPER (GPR30)
Protein Aliases:
Flow-induced endothelial G-protein coupled receptor 1|FEG-1|G protein-coupled estrogen receptor 1|G-protein coupled receptor 30|GPCR-Br|IL8-related receptor DRY12|Lymphocyte-derived G-protein coupled receptor|LYGPR|Membrane estrogen receptor|mER
Accession Number:
NM_001505
UniProt Number:
Q99527
Gene Name:
GPER1
Gene ID:
2852
Gene Aliases:
CEPR|CMKRL2|DRY12|GPER|GPR30
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®