Biological Information
Background Information:
The PathHunter® mCCR6 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL20/MIP-3α) based activation of mCCR6 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged mCCR6 and EA-tagged β-Arrestin 2. Activation of mCCR6 stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein.
Target Class:
GPCR
Family:
Chemokine
Sub Family:
Class A
Coupling:
Gi/Go
Protein Name:
CCR6
Protein Aliases:
CC-CKR-6|CCR-6|Chemokine receptor-like 3|CKR-L3|DRY6|G-protein coupled receptor 29|GPR-CY4|GPRCY4|LARC receptor|CD antigen CD196
Accession Number:
NM_009835.3
UniProt Number:
O54689
Gene Name:
CCR6
Gene ID:
12458
Gene Aliases:
CKR-L3|GPR-CY4|CMKBR6|GPR29|DRY-6|DCR2|BN-1|CD196
Target Species:
Mouse
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®