PathHunter® eXpress CCR2 Total GPCR Internalization Assay

Background Information:

The PathHunter® CCR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. CCL2/MCP-1) based internalization of CCR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged CCR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR2

Protein Aliases:

CC-CKR-2|CCR-2|CCR2|Monocyte chemoattractant protein 1 receptor|MCP-1-R|CD antigen CD192

Accession Number:

NM_001123396

UniProt Number:

P41597

Gene Name:

CCR2

Gene ID:

729230

Gene Aliases:

CC-CKR-2|CKR2|MCP-1-R|CD192|FLJ78302

Target Species:

Human

Cell Background:

U2OS