PathHunter® eXpress EDG1 Total GPCR Internalization Assay

Background Information:

The PathHunter® EDG1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. S1P) based internalization of EDG1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged EDG1 and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDG1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

S1P1

Protein Aliases:

EDG1|Endothelial differentiation G-protein coupled receptor 1|Sphingosine 1-phosphate receptor Edg-1|S1P receptor Edg-1|CD antigen CD363

Accession Number:

NM_001400

UniProt Number:

P21453

Gene Name:

S1PR1

Gene ID:

1901

Gene Aliases:

edg-1|D1S3362|CD363

Target Species:

Human

Cell Background:

HEK293