PathHunter® eXpress GPR35 Activated GPCR Internalization Assay

Background Information:

The PathHunter® GPR35 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. LPA, zaprinast) based internalization of GPR35 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Coupling:

Gi/Go

Protein Name:

GPR35

Protein Aliases:

KYNA receptor

Accession Number:

NM_005301

UniProt Number:

Q9HC97

Gene Name:

GPR35

Gene ID:

2859

Target Species:

Human

Cell Background:

U2OS