PathHunter® GPR35 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® GPR35 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. LPA, zaprinast) based internalization of GPR35 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Coupling:

Gi/Go

Protein Name:

GPR35

Protein Aliases:

KYNA receptor

Accession Number:

NM_005301

UniProt Number:

Q9HC97

Gene Name:

GPR35

Gene ID:

2859

Target Species:

Human

Cell Background:

U2OS