Biological Information
Background Information:
The PathHunter® CCKAR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. cholecystokinin) based activation of CCKAR GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CCKAR and EA-tagged β-Arrestin 2. Activation of CCKAR stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Cholecystokinin
Sub Family:
Class A
Coupling:
Gq
Protein Name:
CCK1 (CCKA)
Protein Aliases:
CCK-AR|Cholecystokinin-1 receptor|CCK1-R
Accession Number:
AY322549
UniProt Number:
P32238
Gene Name:
CCKAR
Gene ID:
886
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
β-Arrestin Isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®