PathHunter® Human BMP-9 (ACVRL1/ACVR2) Stable Cell Line Dimerization Assay (U2OS)

Background Information:

The PathHunter Human BMP-9 (ACVRL1/ACVR2) Stable Cell Line Dimerization Assay (U2OS) utilizes the ACVRL1/ACVR2 Dimerization Cell Line. This assay enables the assessment of ligand (e.g. GDF2/BMP-9)-induced dimerization of ACVRL1/ACVR2 pairs. The included cell line employs the PathHunter Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress ACVRL1 fused to PK and ACVR2 fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10 plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

RSTK

Sub Family:

Protein Ser/Thr

Protein Name:

ALK1

Protein Aliases:

Activin receptor-like kinase 1|ALK-1|TGF-B superfamily receptor type I|TSR-I

Accession Number:

NM_000020.0

UniProt Number:

P37023

Gene Name:

ACVRL1

Gene ID:

94

Gene Aliases:

HHT2|ALK1|HHT|ACVRLK1

Target Species:

Human

Cell Background:

U2OS