PathHunter® Human CD27 Signaling NF-kB Reporter Stable Cell Line Assay (HEK 293)

Background Information:

The PathHunter Human CD27 Signaling NF-kB Reporter Stable Cell Line Assay (HEK 293) utilizes the HEK 293 CD27 NF-kB Signaling Pathway Reporter Cell Line. This engineered cell line monitors the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. It can be used in studying drug candidates (small molecules or biologics) affecting the NF-kB pathway. This cell line enables assessment of NF-kB pathway stimulation based on changes in the expression of a NF-kB response element-driven reporter protein. The included cell line overexpresses CD27, and expresses PL-tagged reporter protein under the control of a NF-kB-inducible transcriptional response element. Pathway activation induces expression of the PL-tagged protein, allowing detection of EFC signal upon addition of EA-containing detection reagent.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10 plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Signaling Pathway

Family:

Tumour Necrosis Factor (TNF)

Protein Name:

CD27

Protein Aliases:

CD27 antigen|T-cell activation antigen S152|TNFRSF7

Accession Number:

NM_001242.5; NM_003998.4

UniProt Number:

P26842

Gene Name:

CD27

Gene ID:

939

Gene Aliases:

S152|Tp55

Target Species:

Human

Cell Background:

HEK293