PathHunter® Human Erythropoietin (EpoR/EpoR) Dimerization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human Erythropoietin (EpoR/EpoR) Dimerization Cell-Based Assay Kit (U2OS) utilizes the PathHunter® U2OS EpoR/EpoR Dimerization Cell Line. This assay enables the assessment of ligand (e.g. erythropoietin)-induced dimerization of EpoR/EpoR. The included cell line employs the PathHunter® Dimerization format using the Enzyme Fragment Complementation (EFC) technology, where the receptor constructs are tagged to enable complementation of the enzyme fragments upon interaction, producing a robust chemiluminescent signal proportional to the dimerization events. The cells overexpress one construct containing EpoR fused to PK and another construct containing EpoR fused to EA, enabling ligand-dependent dimerization measurement upon application of the detection kit.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Cytokine Receptor

Family:

Prolactin Receptor

Protein Name:

EPOR

Accession Number:

NM_000121.3

UniProt Number:

P19235

Gene Name:

EPOR

Gene ID:

2057

Target Species:

Human

Cell Background:

U2OS