PathHunter® Human GPR120 Total GPCR Internalization Cell-Based Assay Kit (HEK 293)

Background Information:

The PathHunter® Human GPR120 Total GPCR Internalization Cell-Based Assay Kit (HEK 293) utilizes the GPR120L Total GPCR internalization cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. free fatty acids) based internalization of GPR120L via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged GPR120L and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, the resulting internalization of the GPR120L receptor to the early endosome leads to production of the EFC signal. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Free Fatty Acid

Sub Family:

Class A

Protein Name:

FFA4 (GPR120)

Protein Aliases:

G-protein coupled receptor 129|G-protein coupled receptor GT01|G-protein coupled receptor PGR4|Omega-3 fatty acid receptor 1

Accession Number:

NM_181745

UniProt Number:

Q5NUL3

Gene Name:

FFAR4

Gene ID:

338557

Gene Aliases:

PGR4

Target Species:

Human

Cell Background:

HEK293