PathHunter® Human GPR173 β-Arrestin Stable Cell Line Assay (HEK 293)

Background Information:

The PathHunter Human GPR173 β-Arrestin Stable Cell Line Assay (HEK 293) utilizes the GPR173 β-Arrestin orphan cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand based activation of GPR173 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged GPR173 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. Please note that this cell line's target receptor is considered an orphan, and orphan receptors typically do not have established control ligands.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Protein Name:

GPR173

Accession Number:

NM_018969

UniProt Number:

Q9NS66

Gene Name:

GPR173

Gene ID:

54328

Gene Aliases:

SREB3

Target Species:

Human

Cell Background:

HEK293