PathHunter® Human Insulin Receptor B (INSRb) Stable Cell Line Internalization Assay (U2OS)

Background Information:

The PathHunter Human Insulin Receptor B (INSRb) Stable Cell Line Internalization Assay (U2OS) utilizes the INSRb Internalization Cell Line. The stable engineered cell line is designed for use in studying molecules targeting RTKs. This cell line enables assessment of RTK activation via detection of INSRb internalization to endosomes. This product uses EFC technology. The included cell line overexpresses PK-tagged INSRb and EA-tagged endosome fusion proteins. Upon ligand-induced activation of the receptor, internalization of the receptor to the endosome leads to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10 plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

IR

Protein Aliases:

CD antigen CD220 [Cleaved into: Insulin receptor subunit alpha; Insulin receptor subunit beta]

Accession Number:

NM_000208

UniProt Number:

P06213

Gene Name:

INSR

Gene ID:

3643

Gene Aliases:

CD220

Target Species:

Human

Cell Background:

U2OS