PathHunter® Human KCNH2(G601S) MEM-EA Stable Cell Line Pharmacotrafficking Assay (U2OS)

Background Information:

The PathHunter Human KCNH2(G601S) MEM-EA Stable Cell Line Pharmacotrafficking Assay (U2OS) utilizes the KCNH2(G601S) MEM-EA Pharmacotrafficking cell line. The stable engineered cell line is designed for use in studying pharmacochaperones targeting KCNH2. This cell line enables assessment of chaperone-based stabilization of KCNH2(G601S) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant KCNH2(G601S), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant KCNH2, it is trafficked to the membrane, producing EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Ion Channel

Family:

Potassium

Sub Family:

Voltage-Gated

Protein Name:

hERG

Protein Aliases:

Ether-a-go-go-related gene potassium channel 1|ERG-1|Eag-related protein 1|Ether-a-go-go-related protein 1|H-ERG|hERG-1|hERG1|Voltage-gated potassium channel subunit Kv11.1

Accession Number:

NM_000238

UniProt Number:

Q12809

Gene Name:

KCNH2

Gene ID:

3757

Gene Aliases:

Kv11.1|HERG|erg1

Target Species:

Human

Cell Background:

U2OS