PathHunter® Human OXGR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human OXGR1 β-Arrestin Cell-Based Assay Kit (CHO-K1) utilizes the Human OXGR1 β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., alpha-ketoglutaric acid) based activation of OXGR1 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged OXGR1 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Oxoglutarate

Sub Family:

Class A

Coupling:

Gq

Protein Name:

OXGR1 (GPR99)

Protein Aliases:

2-oxoglutarate receptor 1|alpha-ketoglutarate receptor 1

Accession Number:

NM_080818.3

UniProt Number:

Q96P68

Gene Name:

OXGR1

Gene ID:

27199

Gene Aliases:

P2RY15|P2Y15|aKGR

Target Species:

Human

Cell Background:

CHO-K1