PathHunter® OPRD1 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® OPRD1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. DADLE/DPDPE) based internalization of OPRD1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged OPRD1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting OPRD1-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

delta (DOP)

Protein Aliases:

DOR-1

Accession Number:

NM_000911

UniProt Number:

P41143

Gene Name:

OPRD1

Gene ID:

4985

Gene Aliases:

OPRD

Target Species:

Human

Cell Background:

U2OS