PathHunter® OPRK1 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® OPRK1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. dynorphin A) based internalization of OPRK1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged OPRK1 and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRK1 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

kappa (KOP)

Protein Aliases:

KOR-1

Accession Number:

AF498922

UniProt Number:

P41145

Gene Name:

OPRK1

Gene ID:

4986

Gene Aliases:

KOR|OPRK

Target Species:

Human

Cell Background:

U2OS