PathHunter® U2OS CD33 Internalization Cell Line

Background Information:

PathHunter® U2OS CD33 Internalization Cell Line is engineered to co-express the ProLink™ (PK) tagged CD33 receptor, and an Enzyme Acceptor (EA) tag localized to the endosomes. Ligand (e.g. Sialic Acid, anti-CD33 antibody) binding to the receptor stimulates receptor internalization, resulting in colocalization of the receptor-PK with the EA in the endosomes, and forcing complementation of the two β galactosidase (β-gal) enzyme fragments (EA and PK). The resulting functional β-gal enzyme hydrolyzes substrate to generate a chemiluminescent signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Checkpoint Receptor

Family:

T Cell Receptor

Protein Name:

CD33

Protein Aliases:

Sialic acid binding Ig-like lectin 3

Accession Number:

NM_001772.3

UniProt Number:

P20138

Gene Name:

CD33

Gene ID:

945

Gene Aliases:

SIGLEC3|SIGLEC-3|p67|FLJ00391|CD33rSiglec

Target Species:

Human

Cell Background:

U2OS