PathHunter® U2OS FGFR4-β-Klotho Bioassay Kit

Background Information:

The PathHunter® FGFR4-β-Klotho Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses FGF-19 induced activation of FGFR4-β-Klotho RTK receptors via detection of SH2 domain protein recruitment. The included cells overexpress ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged co-receptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

FGFR4

Protein Aliases:

CD antigen CD334

Accession Number:

NM_002011

UniProt Number:

P22455

Gene Name:

FGFR4

Gene ID:

2264

Gene Aliases:

JTK2|CD334

Target Species:

Human

Cell Background:

U2OS