Biological Information

Background Information:

The PathHunter® U2OS mINSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged mINSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the mouse version of the protein.

Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

IR-beta

Protein Aliases:

Insulin receptor subunit beta|INSRb

Accession Number:

AGH68804.1

UniProt Number:

M4TKR7

Gene Name:

INSR

Gene ID:

16337

Gene Aliases:

CD220

Target Species:

Mouse

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Functional Activity

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®