PathHunter® UTR2 Total GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® UTR2 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. urotensin II) based internalization of UTR2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses PK-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Urotensin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

UT (GPR14)

Protein Aliases:

G-protein coupled receptor 14|Urotensin II receptor|UR-II-R

Accession Number:

NM_018949

UniProt Number:

Q9UKP6

Gene Name:

UTS2R

Gene ID:

2837

Gene Aliases:

GPR14

Target Species:

Human

Cell Background:

U2OS