Ready-to-Assay A2A Adenosine Receptor Frozen Cells

Background Information:

The Ready-to-Assay A2A Adenosine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. adenosine, NECA) based activation of ADORA2A GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADORA2A alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADORA2A , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Adenosine

Sub Family:

Class A

Protein Name:

A2A

Accession Number:

NM_000675.4

UniProt Number:

P29274

Gene Name:

ADORA2A

Gene ID:

135

Gene Aliases:

RDC8|ADORA2

Target Species:

Human

Cell Background:

HEK293