Ready-to-Assay C5aR Anaphylotoxin Receptor Frozen Cells

Background Information:

The Ready-to-Assay C5aR Anaphylatoxin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress C5AR1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of C5AR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Complement Peptide

Sub Family:

Class A

Protein Name:

C5aR

Protein Aliases:

C5a-R|C5aR|CD antigen CD88

Accession Number:

NM_001736

UniProt Number:

P21730

Gene Name:

C5AR1

Gene ID:

728

Gene Aliases:

C5A|C5AR|CD88

Target Species:

Human

Cell Background:

Chem-1