Ready-to-Assay S1P2 Lysophospholipid Receptor Frozen Cells

Background Information:

The Ready-to-Assay S1P2 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress S1PR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Protein Name:

S1P2

Protein Aliases:

EDG5|Endothelial differentiation G-protein coupled receptor 5|Sphingosine 1-phosphate receptor Edg-5|S1P receptor Edg-5

Accession Number:

NM_004230

UniProt Number:

O95136

Gene Name:

S1PR2

Gene ID:

9294

Gene Aliases:

Gpcr13|H218|AGR16

Target Species:

Human

Cell Background:

Chem-1