Ready-to-Assay VPAC1 Receptor Frozen Cells

Background Information:

The Ready-to-Assay VPAC1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. VIP) based activation of VIPR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress VIPR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of VIPR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

VIP and PACAP

Sub Family:

Class B

Protein Name:

VIP1 (VPAC1)

Protein Aliases:

PVR2|HVR1|RDC1|VIP and PACAP receptor 1|PACAP-R2|pituitary adenylate cyclase-activating polypeptide type II receptor|VIP-R1

Accession Number:

NM_004624

UniProt Number:

P32241

Gene Name:

VIPR1

Gene ID:

7433

Gene Aliases:

VPAC1|RDC1|HVR1|VPAC1R

Target Species:

Human

Cell Background:

Chem-1